Regulatory

Part:BBa_K1400002:Design

Designed by: Dylan Siriwardena   Group: iGEM14_uOttawa   (2014-10-06)


PGAL Modified native pGAL


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 76
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In the scrambling after the TATA box, the same GC content was maintained as with tet binding sites. Mig1 sites were scrambled to allow for grow up in glucose.


Source

This part was generated by modifying the native Gal promoter from Saccharomyces cerevisiae.

References