Regulatory
Part:BBa_K1400002:Design
Designed by: Dylan Siriwardena Group: iGEM14_uOttawa (2014-10-06)
PGAL Modified native pGAL
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 76
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
In the scrambling after the TATA box, the same GC content was maintained as with tet binding sites. Mig1 sites were scrambled to allow for grow up in glucose.
Source
This part was generated by modifying the native Gal promoter from Saccharomyces cerevisiae.